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rabbit anti psd 95  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti psd 95
    Rabbit Anti Psd 95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+psd+95/pm41254409-61-38-42
    Average 86 stars, based on 1 article reviews
    rabbit anti psd 95 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Autophagy controls the hippocampal postsynaptic organization and affects cognition in a mouse model of Fragile X syndrome.
    Article Snippet: .. Primary antibodies used for Western blot include: rabbit anti-LC3-I/II (Novus), rabbit anti-p62 (MBL), rabbit anti-PSD-95 (Cell Signaling), mouse anti-Ubiquitin (Enzo), rabbit anti-ATG7 (Cell Signaling), rabbit anti-Cofilin1 (Cell Signaling), rabbit anti-phospho-Cofilin1-Ser3 (Cell Signaling), rabbit anti-eIF4G1 (Cell Signaling), rabbit anti-CYFIP1 (Millipore), rabbit anti-eIF4E (Cell Signaling), mouse anti-Rac1 (Millipore), mouse anti-puromycin (DSHB), rabbit anti-GAPDH (Cell Signaling) and rabbit anti-β-actin (Sigma). .. Antibodies for mouse anti-PSD-95 (Thermo Fisher Scientific), RFP (Thermo Fisher Scientific), rabbit anti-phospho-Cofilin1-Ser3 (Cell Signaling), rabbit anti-FMRP (Abcam), and eIF4G1 were used for immunocytochemistry with primary antibodies of chicken anti-MAP2 (Millipore) or mouse anti-Tuj-1 (R&D SYSTEMS).

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (CaV3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62- 6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Article Title: Preclinical evaluation of ZL006-05, a new anti-stroke drug with fast-onset antidepressant and anxiolytic effects
    Article Snippet: .. Proteins were analyzed by immunoblotting using rabbit anti-nNOS (1:1000, 61-7000, Thermo), rabbit anti-PSD-95 (1:1000, 34501, Cell Signaling Technology), rabbit anti-CAPON (1:1000, ab 190686, Abcam), rabbit anti-SERT (1:1000, AMT-004, Alomone labs) or rabbit anti-NR2B (1:1000, 21920-1-AP, Proteintech). ..

    Article Title: Autophagy controls the hippocampal postsynaptic organization and affects cognition in a mouse model of Fragile X syndrome
    Article Snippet: .. Primary antibodies used for Western blot include: rabbit anti-LC3-I/II (Novus), rabbit anti-p62 (MBL), rabbit anti-PSD-95 (Cell Signaling), mouse anti-Ubiquitin (Enzo), rabbit anti-ATG7 (Cell Signaling), rabbit anti-Cofilin1 (Cell Signaling), rabbit anti-phospho-Cofilin1-Ser3 (Cell Signaling), rabbit anti-eIF4G1 (Cell Signaling), rabbit anti-CYFIP1 (Millipore), rabbit anti-eIF4E (Cell Signaling), mouse anti-Rac1 (Millipore), mouse anti-puromycin (DSHB), rabbit anti-GAPDH (Cell Signaling) and rabbit anti-β-actin (Sigma). .. Antibodies for mouse anti-PSD-95 (Thermo Fisher Scientific), RFP (Thermo Fisher Scientific), rabbit anti-phospho-Cofilin1-Ser3 (Cell Signaling), rabbit anti-FMRP (Abcam), and eIF4G1 were used for immunocytochemistry with primary antibodies of chicken anti-MAP2 (Millipore) or mouse anti-Tuj-1 (R&D SYSTEMS).

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (Ca V 3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62-6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Blocking Assay:

    Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus.
    Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening.

    Article Title: Progressive Hippocampal Neuroarchitecture Changes in the 5×FAD Alzheimer’s Disease Mouse Model
    Article Snippet: To block nonspecific binding, membranes were incubated at RT for 1 h in PBS (1X; diluted from 10X stock, 502B828, Samchun Pure Chemical Co., Ltd.) with 0.1% (v/v) Tween-20 (#P2287, SigmaAldrich; PBS-T; pH 7.4) containing 1% (v/v) normal goat serum (NGS; #S-1000, Vector Laboratories, Burlingame, CA, USA) and 0.5% (v/v) bovine serum albumin (BSA; #A9647, Sigma-Aldrich). .. Membranes were then incubated overnight at 4 °C with primary antibodies diluted in blocking solution and then left at RT for 1 h. Primary antibodies included mouse anti-Aβ (1-16) (clone 6E10, 1:1000; #803001, BioLegend, San Diego, CA, USA), rabbit anti-p-Tau (Thr181) (1:1000; #12885S, Cell Signaling Technology, Danvers, MA, USA), mouse anti-glial fibrillary acidic protein (GFAP, 1:1000; #3670, Cell Signaling Technology), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1, 1:1000; #016-20001, Fujifilm Wako, Osaka, Japan), rabbit anti-PSD-95 (1:1000; #2507, Cell Signaling Technology), and rabbit anti-activity-regulated cytoskeleton-associated protein (Arc; 1:1000; #ab183183, Abcam, Cambridge, UK). .. Subsequent to the primary antibody incubation, membranes were incubated for 2 h with either a horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:5000; #31460, Thermo Fisher Scientific, Waltham, MA, USA) or mouse secondary antibody (1:5000; #31181, Thermo Fisher Scientific).

    Incubation:

    Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus.
    Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening.

    Article Title: Progressive Hippocampal Neuroarchitecture Changes in the 5×FAD Alzheimer’s Disease Mouse Model
    Article Snippet: To block nonspecific binding, membranes were incubated at RT for 1 h in PBS (1X; diluted from 10X stock, 502B828, Samchun Pure Chemical Co., Ltd.) with 0.1% (v/v) Tween-20 (#P2287, SigmaAldrich; PBS-T; pH 7.4) containing 1% (v/v) normal goat serum (NGS; #S-1000, Vector Laboratories, Burlingame, CA, USA) and 0.5% (v/v) bovine serum albumin (BSA; #A9647, Sigma-Aldrich). .. Membranes were then incubated overnight at 4 °C with primary antibodies diluted in blocking solution and then left at RT for 1 h. Primary antibodies included mouse anti-Aβ (1-16) (clone 6E10, 1:1000; #803001, BioLegend, San Diego, CA, USA), rabbit anti-p-Tau (Thr181) (1:1000; #12885S, Cell Signaling Technology, Danvers, MA, USA), mouse anti-glial fibrillary acidic protein (GFAP, 1:1000; #3670, Cell Signaling Technology), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1, 1:1000; #016-20001, Fujifilm Wako, Osaka, Japan), rabbit anti-PSD-95 (1:1000; #2507, Cell Signaling Technology), and rabbit anti-activity-regulated cytoskeleton-associated protein (Arc; 1:1000; #ab183183, Abcam, Cambridge, UK). .. Subsequent to the primary antibody incubation, membranes were incubated for 2 h with either a horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:5000; #31460, Thermo Fisher Scientific, Waltham, MA, USA) or mouse secondary antibody (1:5000; #31181, Thermo Fisher Scientific).

    Gentle:

    Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus.
    Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening.

    Slice Preparation:

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (CaV3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62- 6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (Ca V 3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62-6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Immunohistochemistry:

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (CaV3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62- 6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (Ca V 3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62-6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Cell Culture:

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (CaV3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62- 6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (Ca V 3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62-6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Immunocytochemistry:

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice.
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (CaV3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62- 6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.

    Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice
    Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), rabbit anti-PSD-95 (1:1000 for WB, Cell Signaling #3450), mouse anti-RGS14 (1:1000 for WB, Antibodies Inc # 75-170-020), rabbit anti-MAGEL2 (1:1000 for WB, Bioss Antibodies bs-6828R), and mouse anti-CACNA1G (Ca V 3.1, 1:1000 for WB, Novus Biologicals #NBP2-59322). .. The following secondary antibodies were used: IgG-HRP for WB [1:10000; Thermo Fisher, mouse (#62-6520) and rabbit (#31460)] and Alexa Fluor 555 [1:500, Thermo Fisher, rabbit (#A32732)] for ICC and IHC.



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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
    Rabbit Monoclonal Anti Psd95 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Image Search Results


    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Journal: Frontiers in Endocrinology

    Article Title: Dorzagliatin shows potential in preventing cognitive impairment in diabetes: evidence from Mendelian randomization analysis and animal study

    doi: 10.3389/fendo.2025.1755359

    Figure Lengend Snippet: Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Article Snippet: The membranes were blocked with 1×TBST and 5% BSA (tank blotting) or 5% skim milk (Semi-dry blotting) for 1 hour at room temperature and then incubated overnight at 4°C with following primary antibodies, respectively: mouse monoclonal anti-GluN1 antibody (Millipore, Cat. No: 05-432); rabbit polyclonal anti-GluN2A antibody (NOVUS, Cat. No: NB300-105); rabbit monoclonal anti-GLUT1 antibody (Abcam, Cat. No: ab115730); rabbit polyclonal anti-GLUT3 antibody (Bioss, Cat. No: bs-1207R); rabbit polyclonal anti-IR antibody (Abcam, Cat. No: ab137747); rabbit monoclonal anti-PSD95 antibody (Abcam, Cat. No: ab238135).

    Techniques: Western Blot, Control, Two Tailed Test