rabbit anti psd 95 (Cell Signaling Technology Inc)
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Rabbit Anti Psd 95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psd+95/pm41254409-61-38-42
Average 86 stars, based on 1 article reviews
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Western Blot:Article Title: Autophagy controls the hippocampal postsynaptic organization and affects cognition in a mouse model of Fragile X syndrome. Article Snippet: .. Primary antibodies used for Western blot include: rabbit anti-LC3-I/II (Novus), rabbit anti-p62 (MBL), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), Article Title: Preclinical evaluation of ZL006-05, a new anti-stroke drug with fast-onset antidepressant and anxiolytic effects Article Snippet: .. Proteins were analyzed by immunoblotting using rabbit anti-nNOS (1:1000, 61-7000, Thermo), Article Title: Autophagy controls the hippocampal postsynaptic organization and affects cognition in a mouse model of Fragile X syndrome Article Snippet: .. Primary antibodies used for Western blot include: rabbit anti-LC3-I/II (Novus), rabbit anti-p62 (MBL), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), Blocking Assay:Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus. Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening. Article Title: Progressive Hippocampal Neuroarchitecture Changes in the 5×FAD Alzheimer’s Disease Mouse Model Article Snippet: To block nonspecific binding, membranes were incubated at RT for 1 h in PBS (1X; diluted from 10X stock, 502B828, Samchun Pure Chemical Co., Ltd.) with 0.1% (v/v) Tween-20 (#P2287, SigmaAldrich; PBS-T; pH 7.4) containing 1% (v/v) normal goat serum (NGS; #S-1000, Vector Laboratories, Burlingame, CA, USA) and 0.5% (v/v) bovine serum albumin (BSA; #A9647, Sigma-Aldrich). .. Membranes were then incubated overnight at 4 °C with primary antibodies diluted in blocking solution and then left at RT for 1 h. Primary antibodies included mouse anti-Aβ (1-16) (clone 6E10, 1:1000; #803001, BioLegend, San Diego, CA, USA), rabbit anti-p-Tau (Thr181) (1:1000; #12885S, Cell Signaling Technology, Danvers, MA, USA), mouse anti-glial fibrillary acidic protein (GFAP, 1:1000; #3670, Cell Signaling Technology), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1, 1:1000; #016-20001, Fujifilm Wako, Osaka, Japan), Incubation:Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus. Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening. Article Title: Progressive Hippocampal Neuroarchitecture Changes in the 5×FAD Alzheimer’s Disease Mouse Model Article Snippet: To block nonspecific binding, membranes were incubated at RT for 1 h in PBS (1X; diluted from 10X stock, 502B828, Samchun Pure Chemical Co., Ltd.) with 0.1% (v/v) Tween-20 (#P2287, SigmaAldrich; PBS-T; pH 7.4) containing 1% (v/v) normal goat serum (NGS; #S-1000, Vector Laboratories, Burlingame, CA, USA) and 0.5% (v/v) bovine serum albumin (BSA; #A9647, Sigma-Aldrich). .. Membranes were then incubated overnight at 4 °C with primary antibodies diluted in blocking solution and then left at RT for 1 h. Primary antibodies included mouse anti-Aβ (1-16) (clone 6E10, 1:1000; #803001, BioLegend, San Diego, CA, USA), rabbit anti-p-Tau (Thr181) (1:1000; #12885S, Cell Signaling Technology, Danvers, MA, USA), mouse anti-glial fibrillary acidic protein (GFAP, 1:1000; #3670, Cell Signaling Technology), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1, 1:1000; #016-20001, Fujifilm Wako, Osaka, Japan), Gentle:Article Title: Eye Opening Primes Synaptic Maturation by Enhancing AMPA Receptor-Mediated Transmission and Spinogenesis in the Developing Superior Colliculus. Article Snippet: Eye opening represents a critical milestone in postnatal visual development, triggering experience-dependent plasticity that refines neural circuits across visual pathways.. While synaptic maturation in the thalamocortical system has been extensively studied, how eye opening shapes synaptic development in the superior colliculus (SC) – a major subcortical hub mediating rapid orienting and motion detection – remains poorly understood.. Here, we combine electrophysiological, biochemical, and ultrastructural approaches to investigate experience-promoted synaptic development in the mouse SC following eye opening. Slice Preparation:Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), Immunohistochemistry:Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), Cell Culture:Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), Immunocytochemistry:Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [42]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75- 251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbitanti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit antiNeurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1- AP), Article Title: Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice Article Snippet: Blots were visualized using the Azure Radiance Plus chemiluminescence detection system (Azure Biosystems #AC2103) on the Sapphire Biomolecular Imager (Azure Biosystems) and analyzed using NIH Fiji (ImageJ, RRID:SCR_002285) [ ]. .. Primary antibodies to the following proteins were used: rabbit anti-KIAA2022 [1:100 (brain slice for IHC) and 1:300 (cultured neurons for ICC), Sigma #HPA000407], rabbit anti-KIAA2022 (1:750 for WB; Biorbyt Orb312213), rabbit anti-β-tubulin III (1:1000 for WB, Sigma-Aldrich T2200), rabbit anti-GluA1 (1:1000 for WB, Homemade), mouse anti-SynDIG1 (1:1000 for WB, Antibodies Inc #75-251), rabbit anti-TrkB (1:1000 for WB, Cell Signaling #4603), rabbit-anti BAIAP3 (1:1000 for WB, Synaptic Systems 256 003), rabbit-anti Cerebellin-1 (1:1000 for WB; Boster Bio A09176-1), rabbit anti-Neurotensin (1:1000 for WB, Abcam #ab233107), rabbit anti-Filip1 N-terminal (1:1000 for WB, Aviva Systems Biology #ARP79028_P050), rabbit anti-NMDAR1 (1:1000 for WB, Sigma #G8913), rabbit anti-GLRA1 (1:1000 for WB, Proteintech #17951-1-AP), |
